A team of researchers from the State University of Campinas (Unicamp) and the University of São Paulo (USP) were able to explain how human proteins work with SARS-CoV-2 protein, revealing one of the ways the virus “collects” body cells to replicate.
It’s a test in vitro, was able to inhibit the interaction between the molecules of the virus and those entering the body through treatment, reducing the number of viruses by 15% to 20%. The study in question was published in a scientific journal Frontiers in Cellular and Infection Microbiology.
Proteins, viruses and DNA
The protein required by the PCNA – Proliferating Cell Nuclear Antigen – binds to the SARS-CoV-2 protein (matrix), one of the viral molecules and provides its own form. The study of this interaction demonstrates one way in which microbes use the cell to replicate in our body.
Laboratory studies sought to demonstrate how the presence of M protein binds PCNA – involved in DNA repair – from cell nucleus, where it should be, to the cytoplasm, where organelles with different cell functions are found.
One way to ensure interaction was to use a component that inhibits the migration of proteins to the cytoplasm: all drugs that inhibit PCNA and further inhibit HIV infection by 15% to 20%, compared to unaffected cells.
Scientists say, yes, the reduction may not be necessary if we consider the development of anti-retroviral treatment for SARS-CoV-2, but as the main function of this research is to show interaction and evaluation as a therapeutic approach. instead of future treatment, the findings seemed a success.
Another method of studying this phenomenon was the analysis of lung tissue from the autopsies of patients killed by Covid-19. In them, a significant increase in PCNA and gammaH2AX proteins was observed, indicating DNA damage – enhancing other laboratory results. According to scientists, it is evidence of some side effects of covid fever.
Along with the proteins E and S, M is one of those found in the membranes that surround SARS-CoV-2, which is also more numerous and one of the four genes, that is, the compounds that make up the virus. This makes it a target for medicines such as vaccines and medicines. S (spike) proteins are known, for example, to bind to human receptors, and have been resistant to modern vaccines.
PCNA often learns more about cancer, and less is known about its role in HIV: hence the importance of recent Brazilian research. It is suggested that the following steps may, for example, confirm the findings of animal experiments.
Source: Frontiers in Cellular and Infection Microbiology